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| Registros recuperados : 39 | |
| 2. |  | ANACKER, L. F.; MOTA, M. S.; SCHAFER, J. T.; ARDUIM, G. S.; GOMES, C. B.; MOURA, A. B. Produção de compostos voláteis por bactérias biocontroladoras de Mesocriconema xenoplax E Meloidogyne incógnita. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 44., 2011, Bento Gonçalves. Anais... Lavras, MG: SBF, 2011. v. 36, p. 446.| Biblioteca(s): Embrapa Clima Temperado. |
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| 3. | | BECKER, C. E.; GOMES, C. B.; SCHAFER, J. T.; BRETANHA, J. da R. R. Potencial de rizobactérias no biocontrole de Mesocriconema xenoplax. In: CONGRESSO BRASILEIRO DE DEFENSIVOS AGRÍCOLAS NATURAIS, 7., 2015, Pelotas. Anais... Pelotas: Embrapa Clima Temperado, Brasília, DF: Embrapa, 2015. 1 CD-ROM.| Biblioteca(s): Embrapa Clima Temperado. |
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| 4. | | BELLÉ, C.; BRUM, D.; GROTH, M. Z.; BARROS, D. R.; KASPARY, T. E.; SCHAFER, J. T.; GOMES, C. B. First Report of Meloidogyne luci Parasitizing Glycine max in Brazil. Plant Disease, v. 100, n. 10, p. 2174, Oct. 2016.| Biblioteca(s): Embrapa Clima Temperado. |
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| 5. | | BRUM, D.; GOMES, C. B.; MEDINA, I. L.; SCHAFER, J. T.; SOMAVILLA, L. Potencial de rizobactérias na colonização radicular e biocontrole do nematoide das galhas (Meloidogyne graminicola) em arroz de sequeiro. In: CONGRESSO BRASILEIRO DE DEFENSIVOS AGRÍCOLAS NATURAIS, 7., 2015, Pelotas. Anais... Pelotas: Embrapa Clima Temperado, Brasília, DF: Embrapa, 2015. 1 CD-ROM.| Biblioteca(s): Embrapa Clima Temperado. |
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| 7. | | EICHOLZ, E. D.; GOMES, C. B.; CORREA, B. O.; SCHAFER, J. T. Efeito da microbiolização de sementes com rizobactérias na qualidade sanitária e produção da mamona. In: CONGRESSO BRASILEIRO DE DEFENSIVOS AGRÍCOLAS NATURAIS, 7., 2015, Pelotas. Anais... Pelotas: Embrapa Clima Temperado, Brasília, DF: Embrapa, 2015. 1 CD-ROM.| Biblioteca(s): Embrapa Clima Temperado. |
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| 8. | | FISS, A. V.; LIMA, C. V.; SCHAFER, J. T.; DIEL, M. I.; MARTINAZZO, R.; SILVEIRA, C. A. P.; BAMBERG, A. L.; BARBIERI, R. L.; GOMES, C. B. Promoção de crescimento e biocontrole de meloidogyne incognita em pimenta´dedo-de-moça´por rizobacterias em condições de casa de vegetação e campo. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO DA EMBRAPA CLIMA TEMPERADO, 5., 2014, Pelotas. Qual o papel da ciência na agricultura familiar?: anais. Pelotas: Embrapa Clima Temperado, 2015. 120 p.| Biblioteca(s): Embrapa Clima Temperado. |
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| 9. | | GOMES, C. B.; SCHAFER, J. T.; BELLÉ, C.; ARAÚJO FILHO, J. V. de. Emprego de leguminosas no manejo de fitonematoides em espécies frutíferas. In: MARTINS, C. R.; GOMES, C. B.; BELLÉ, C.; MEDEIROS, F. S.; CARVALHO, F. L. C.; CARDOSO, J. H.; ARAUJO FILHO, J. V. de; SCHAFER, J. T.; WOLFF, L. F.; BILHARVA, M. G. (Ed.). Leguminosas na fruticultura: uso e integração em propriedades familiares do sul do Brasil. Pelotas: Embrapa Clima Temperado:Brasília, DF: Embrapa, 2019. 66 p. p. 47-66| Biblioteca(s): Embrapa Clima Temperado. |
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| 10. | | LIMA, C. V.; FISS, A. V.; LIMA-MEDINA, I.; WILLE, C. N.; SCHAFER, J. T; GOMES, C. B. Solarização, biofumigação e uso de rizobactérias no controle de Meloidogyne incognita em pimenta. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 47.; SIMPÓSIO BRASILEIRO DE MOFO BRANCO, 2014, Londrina. Desafios futuros: anais. Londrina: Sociedade Brasileira de Fitopatologia, 2014. 1 CD-ROM.| Biblioteca(s): Embrapa Clima Temperado. |
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| 11. | | LIMA-MEDINA, I.; SCHAFER, J. T.; GOMES, C. B. Reaction of potato cultivars to Meloidogyne Hapla and M. Morocciensis. Journal of Nematology, v. 46, n. 2, p. 195, June 2014. Edição dos Proceedings do 6th International Congress of Nematology, Cape Town, South Africa, May 2014.| Biblioteca(s): Embrapa Clima Temperado. |
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| 12. | | LIMA-MEDINA, I.; SCHAFER, J. T.; GOMES, C. B.; VIZZOTTO, M.; KROLOW, A. C. R.; CARNEIRO, R. M. D. G.; CORREA, V. Aggressiveness of meloidogyne javanica populations on commercial potato cultivars. Journal of Nematology, v. 46, n. 2, p. 194-195, June 2014. Edição dos Proceedings do 6th International Congress of Nematology, Cape Town, South Africa, May 2014.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 13. | | LIMA-MEDINA, I.; SCHAFER, J. T.; GOMES, C. B.; VIZZOTTO, M.; KROLOW, A. C. R.; CARNEIRO, R. M. D. G.; CORREA, V. Aggressiveness of meloidogyne javanica populations on commercial potato cultivars. Journal of Nematology, v. 46, n. 2, p. 194-195, June 2014. Edição dos Proceedings do 6th International Congress of Nematology, Cape Town, South Africa, May 2014.| Biblioteca(s): Embrapa Clima Temperado. |
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| 14. | | MARTINS, S. O.; CALSIN, C. P. M.; MOURA, A. B.; FILHO, J. V. A.; RODRIGUES, W. S.; SILVA, S. D. A.; SCHAFER, J. T.; GOMES, C. B.; PEREIRA, M. M. Promoção de crescimento e biocontrole de fitonematoides por rizobactérias em cana-de-açúcar. In: CONGRESSO BRASILEIRO DE NEMATOLOGIA, 37., 2022, Ribeirão Preto. Anais... [São Paulo: Sociedade Brasileira de Nematologia, 2022]. p. 108.| Biblioteca(s): Embrapa Clima Temperado. |
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| 16. | | PACHECO, D.; CASA-COILA, V. H.; SCHAFER, J. T.; BELLÉ, C.; BRUM, D. de; GOMES, C. B. Efeito nematicida e nematostático de rizobactérias sobre Pratylenchus brachyurus. In: CONGRESSO BRASILEIRO DE DEFENSIVOS AGRÍCOLAS NATURAIS, 7., 2015, Pelotas. Anais... Pelotas: Embrapa Clima Temperado, Brasília, DF: Embrapa, 2015. 1 CD-ROM.| Biblioteca(s): Embrapa Clima Temperado. |
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| 19. | | ROSA, A. P. S. A. da; CHEVARRIA, V. V.; SCHÃFER, J. T.; MARTINS, J. F. da S. Efeito da época de semeadura, precipitação e temperatura sobre sobre a população de Spodoptera frugiperda (J. E. SMITH, 1797) (Lepidoptera: Noctuidae) em áreas de terras baixas. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008, Uberlândia. Ciência, tecnologia e inovação: anais... Uberlândia: Sociedade Entomológica do Brasil, 2008. 1 CD-ROM.| Biblioteca(s): Embrapa Clima Temperado. |
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| Registros recuperados : 39 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
07/11/2014 |
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Data da última atualização: |
23/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
RODRIGUES, T. B.; KHAJURIA, C.; WANG, H.; MATZ, N.; CARDOSO, D. C.; VALICENTE, F. H.; ZHOU, X.; SIEGFRIED, B. |
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Afiliação: |
FERNANDO HERCOS VALICENTE, CNPMS. |
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Título: |
Validation of reference housekeeping genes for gene expression studies in western corn rootworm (Diabrotica virgifera virgifera). |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Plos One, San Francisco,v. 9, n. 10, p. 1-8, Oct. 2014. |
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DOI: |
10.1371/journal.pone.0109825 |
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Idioma: |
Inglês |
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Conteúdo: |
Quantitative Real-time PCR (qRT-PCR) is a powerful technique to investigate comparative gene expression. In general, normalization of results using a highly stable housekeeping gene (HKG) as an internal control is recommended and necessary. However, there are several reports suggesting that regulation of some HKGs is affected by different conditions. The western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), is a serious pest of corn in the United States and Europe. The expression profile of target genes related to insecticide exposure, resistance, and RNA interference has become an important experimental technique for study of western corn rootworms; however, lack of information on reliable HKGs under different conditions makes the interpretation of qRT-PCR results difficult. In this study, four distinct algorithms (Genorm, NormFinder, BestKeeper and delta-CT) and five candidate HKGs to genes of reference (b-actin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; b-tubulin; RPS9, ribosomal protein S9; EF1a, elongation factor-1a) were evaluated to determine the most reliable HKG under different experimental conditions including exposure to dsRNA and Bt toxins and among different tissues and developmental stages. Although all the HKGs tested exhibited relatively stable expression among the different treatments, some differences were noted. Among the five candidate reference genes evaluated, b-actin exhibited highly stable expression among different life stages. RPS9 exhibited the most similar pattern of expression among dsRNA treatments, and both experiments indicated that EF1a was the second most stable gene. EF1a was also the most stable for Bt exposure and among different tissues. These results will enable researchers to use more accurate and reliable normalization of qRT-PCR data in WCR experiments. MenosQuantitative Real-time PCR (qRT-PCR) is a powerful technique to investigate comparative gene expression. In general, normalization of results using a highly stable housekeeping gene (HKG) as an internal control is recommended and necessary. However, there are several reports suggesting that regulation of some HKGs is affected by different conditions. The western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), is a serious pest of corn in the United States and Europe. The expression profile of target genes related to insecticide exposure, resistance, and RNA interference has become an important experimental technique for study of western corn rootworms; however, lack of information on reliable HKGs under different conditions makes the interpretation of qRT-PCR results difficult. In this study, four distinct algorithms (Genorm, NormFinder, BestKeeper and delta-CT) and five candidate HKGs to genes of reference (b-actin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; b-tubulin; RPS9, ribosomal protein S9; EF1a, elongation factor-1a) were evaluated to determine the most reliable HKG under different experimental conditions including exposure to dsRNA and Bt toxins and among different tissues and developmental stages. Although all the HKGs tested exhibited relatively stable expression among the different treatments, some differences were noted. Among the five candidate reference genes evaluated, b-actin exhibited highly stable expression a... Mostrar Tudo |
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Thesagro: |
Gene; Genética; Lagarta; Praga de planta. |
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Thesaurus NAL: |
Plant pests. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/111142/1/Validation-reference.pdf
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Marc: |
LEADER 02688naa a2200277 a 4500
001 1999434
005 2017-05-23
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0109825$2DOI
100 1 $aRODRIGUES, T. B.
245 $aValidation of reference housekeeping genes for gene expression studies in western corn rootworm (Diabrotica virgifera virgifera).$h[electronic resource]
260 $c2014
520 $aQuantitative Real-time PCR (qRT-PCR) is a powerful technique to investigate comparative gene expression. In general, normalization of results using a highly stable housekeeping gene (HKG) as an internal control is recommended and necessary. However, there are several reports suggesting that regulation of some HKGs is affected by different conditions. The western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), is a serious pest of corn in the United States and Europe. The expression profile of target genes related to insecticide exposure, resistance, and RNA interference has become an important experimental technique for study of western corn rootworms; however, lack of information on reliable HKGs under different conditions makes the interpretation of qRT-PCR results difficult. In this study, four distinct algorithms (Genorm, NormFinder, BestKeeper and delta-CT) and five candidate HKGs to genes of reference (b-actin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; b-tubulin; RPS9, ribosomal protein S9; EF1a, elongation factor-1a) were evaluated to determine the most reliable HKG under different experimental conditions including exposure to dsRNA and Bt toxins and among different tissues and developmental stages. Although all the HKGs tested exhibited relatively stable expression among the different treatments, some differences were noted. Among the five candidate reference genes evaluated, b-actin exhibited highly stable expression among different life stages. RPS9 exhibited the most similar pattern of expression among dsRNA treatments, and both experiments indicated that EF1a was the second most stable gene. EF1a was also the most stable for Bt exposure and among different tissues. These results will enable researchers to use more accurate and reliable normalization of qRT-PCR data in WCR experiments.
650 $aPlant pests
650 $aGene
650 $aGenética
650 $aLagarta
650 $aPraga de planta
700 1 $aKHAJURIA, C.
700 1 $aWANG, H.
700 1 $aMATZ, N.
700 1 $aCARDOSO, D. C.
700 1 $aVALICENTE, F. H.
700 1 $aZHOU, X.
700 1 $aSIEGFRIED, B.
773 $tPlos One, San Francisco,v. 9$gn. 10, p. 1-8, Oct. 2014.
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Embrapa Milho e Sorgo (CNPMS) |
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